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Separate Developmental Programs for HLA-A and -B Cell Surface Expression during Differentiation from Embryonic Stem Cells to Lymphocytes, Adipocytes and Osteoblasts

A major problem of allogeneic stem cell therapy is immunologically mediated graft rejection. HLA class I A, B, and Cw antigens are crucial factors, but little is known of their respective expression on stem cells and their progenies. We have recently shown that locus-specific expression (HLA-A, but...

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Autores principales: Sabir, Hardee J., Nehlin, Jan O., Qanie, Diyako, Harkness, Linda, Prokhorova, Tatyana A., Blagoev, Blagoy, Kassem, Moustapha, Isa, Adiba, Barington, Torben
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3548781/
https://www.ncbi.nlm.nih.gov/pubmed/23349864
http://dx.doi.org/10.1371/journal.pone.0054366
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author Sabir, Hardee J.
Nehlin, Jan O.
Qanie, Diyako
Harkness, Linda
Prokhorova, Tatyana A.
Blagoev, Blagoy
Kassem, Moustapha
Isa, Adiba
Barington, Torben
author_facet Sabir, Hardee J.
Nehlin, Jan O.
Qanie, Diyako
Harkness, Linda
Prokhorova, Tatyana A.
Blagoev, Blagoy
Kassem, Moustapha
Isa, Adiba
Barington, Torben
author_sort Sabir, Hardee J.
collection PubMed
description A major problem of allogeneic stem cell therapy is immunologically mediated graft rejection. HLA class I A, B, and Cw antigens are crucial factors, but little is known of their respective expression on stem cells and their progenies. We have recently shown that locus-specific expression (HLA-A, but not -B) is seen on some multipotent stem cells, and this raises the question how this is in other stem cells and how it changes during differentiation. In this study, we have used flow cytometry to investigate the cell surface expression of HLA-A and -B on human embryonic stem cells (hESC), human hematopoietic stem cells (hHSC), human mesenchymal stem cells (hMSC) and their fully-differentiated progenies such as lymphocytes, adipocytes and osteoblasts. hESC showed extremely low levels of HLA-A and no -B. In contrast, multipotent hMSC and hHSC generally expressed higher levels of HLA-A and clearly HLA-B though at lower levels. IFNγ induced HLA-A to very high levels on both hESC and hMSC and HLA-B on hMSC. Even on hESC, a low expression of HLA-B was achieved. Differentiation of hMSC to osteoblasts downregulated HLA-A expression (P = 0.017). Interestingly HLA class I on T lymphocytes differed between different compartments. Mature bone marrow CD4(+) and CD8(+) T cells expressed similar HLA-A and -B levels as hHSC, while in the peripheral blood they expressed significantly more HLA-B7 (P = 0.0007 and P = 0.004 for CD4(+) and CD8(+) T cells, respectively). Thus different HLA loci are differentially regulated during differentiation of stem cells.
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spelling pubmed-35487812013-01-24 Separate Developmental Programs for HLA-A and -B Cell Surface Expression during Differentiation from Embryonic Stem Cells to Lymphocytes, Adipocytes and Osteoblasts Sabir, Hardee J. Nehlin, Jan O. Qanie, Diyako Harkness, Linda Prokhorova, Tatyana A. Blagoev, Blagoy Kassem, Moustapha Isa, Adiba Barington, Torben PLoS One Research Article A major problem of allogeneic stem cell therapy is immunologically mediated graft rejection. HLA class I A, B, and Cw antigens are crucial factors, but little is known of their respective expression on stem cells and their progenies. We have recently shown that locus-specific expression (HLA-A, but not -B) is seen on some multipotent stem cells, and this raises the question how this is in other stem cells and how it changes during differentiation. In this study, we have used flow cytometry to investigate the cell surface expression of HLA-A and -B on human embryonic stem cells (hESC), human hematopoietic stem cells (hHSC), human mesenchymal stem cells (hMSC) and their fully-differentiated progenies such as lymphocytes, adipocytes and osteoblasts. hESC showed extremely low levels of HLA-A and no -B. In contrast, multipotent hMSC and hHSC generally expressed higher levels of HLA-A and clearly HLA-B though at lower levels. IFNγ induced HLA-A to very high levels on both hESC and hMSC and HLA-B on hMSC. Even on hESC, a low expression of HLA-B was achieved. Differentiation of hMSC to osteoblasts downregulated HLA-A expression (P = 0.017). Interestingly HLA class I on T lymphocytes differed between different compartments. Mature bone marrow CD4(+) and CD8(+) T cells expressed similar HLA-A and -B levels as hHSC, while in the peripheral blood they expressed significantly more HLA-B7 (P = 0.0007 and P = 0.004 for CD4(+) and CD8(+) T cells, respectively). Thus different HLA loci are differentially regulated during differentiation of stem cells. Public Library of Science 2013-01-18 /pmc/articles/PMC3548781/ /pubmed/23349864 http://dx.doi.org/10.1371/journal.pone.0054366 Text en © 2013 Sabir et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Sabir, Hardee J.
Nehlin, Jan O.
Qanie, Diyako
Harkness, Linda
Prokhorova, Tatyana A.
Blagoev, Blagoy
Kassem, Moustapha
Isa, Adiba
Barington, Torben
Separate Developmental Programs for HLA-A and -B Cell Surface Expression during Differentiation from Embryonic Stem Cells to Lymphocytes, Adipocytes and Osteoblasts
title Separate Developmental Programs for HLA-A and -B Cell Surface Expression during Differentiation from Embryonic Stem Cells to Lymphocytes, Adipocytes and Osteoblasts
title_full Separate Developmental Programs for HLA-A and -B Cell Surface Expression during Differentiation from Embryonic Stem Cells to Lymphocytes, Adipocytes and Osteoblasts
title_fullStr Separate Developmental Programs for HLA-A and -B Cell Surface Expression during Differentiation from Embryonic Stem Cells to Lymphocytes, Adipocytes and Osteoblasts
title_full_unstemmed Separate Developmental Programs for HLA-A and -B Cell Surface Expression during Differentiation from Embryonic Stem Cells to Lymphocytes, Adipocytes and Osteoblasts
title_short Separate Developmental Programs for HLA-A and -B Cell Surface Expression during Differentiation from Embryonic Stem Cells to Lymphocytes, Adipocytes and Osteoblasts
title_sort separate developmental programs for hla-a and -b cell surface expression during differentiation from embryonic stem cells to lymphocytes, adipocytes and osteoblasts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3548781/
https://www.ncbi.nlm.nih.gov/pubmed/23349864
http://dx.doi.org/10.1371/journal.pone.0054366
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