Method to quantify live and dead cells in multi-species oral biofilm by real-time PCR with propidium monoazide

Real-time PCR (qPCR) is a widely used technique in analysing environmental and clinical microbiological samples. However, its main limitation was its inability to discriminate between live and dead cells. Recently, propidium monoazide (PMA) together with qPCR has been used to overcome this problem,...

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Detalles Bibliográficos
Autores principales: Àlvarez, Gerard, González, Marta, Isabal, Sergio, Blanc, Vanessa, León, Rubén
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2013
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3549832/
https://www.ncbi.nlm.nih.gov/pubmed/23289803
http://dx.doi.org/10.1186/2191-0855-3-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3549832/
https://www.ncbi.nlm.nih.gov/pubmed/23289803
http://dx.doi.org/10.1186/2191-0855-3-1

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