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Method to quantify live and dead cells in multi-species oral biofilm by real-time PCR with propidium monoazide
Real-time PCR (qPCR) is a widely used technique in analysing environmental and clinical microbiological samples. However, its main limitation was its inability to discriminate between live and dead cells. Recently, propidium monoazide (PMA) together with qPCR has been used to overcome this problem,...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3549832/ https://www.ncbi.nlm.nih.gov/pubmed/23289803 http://dx.doi.org/10.1186/2191-0855-3-1 |