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Construction of Improved Tools for Protein Localization Studies in Streptococcus pneumoniae

We have constructed a set of plasmids that allow efficient expression of both N- and C-terminal fusions of proteins of interest to fluorescent proteins mCherry, Citrine, CFP and GFP in the Gram-positive pathogen Streptococcus pneumoniae. In order to improve expression of the fluorescent fusions to l...

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Detalles Bibliográficos
Autores principales: Henriques, Mafalda X., Catalão, Maria João, Figueiredo, Joana, Gomes, João Paulo, Filipe, Sergio R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3551898/
https://www.ncbi.nlm.nih.gov/pubmed/23349996
http://dx.doi.org/10.1371/journal.pone.0055049