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Construction of Improved Tools for Protein Localization Studies in Streptococcus pneumoniae

We have constructed a set of plasmids that allow efficient expression of both N- and C-terminal fusions of proteins of interest to fluorescent proteins mCherry, Citrine, CFP and GFP in the Gram-positive pathogen Streptococcus pneumoniae. In order to improve expression of the fluorescent fusions to l...

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Detalles Bibliográficos
Autores principales:	Henriques, Mafalda X., Catalão, Maria João, Figueiredo, Joana, Gomes, João Paulo, Filipe, Sergio R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2013
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3551898/ https://www.ncbi.nlm.nih.gov/pubmed/23349996 http://dx.doi.org/10.1371/journal.pone.0055049

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