Structure and dynamics of a primordial catalytic fold generated by in vitro evolution

Engineering functional protein scaffolds capable of carrying out chemical catalysis is a major challenge in enzyme design. Starting from a non-catalytic protein scaffold, we recently generated a novel RNA ligase by in vitro directed evolution. This artificial enzyme lost its original fold and adopte...

Descripción completa

Detalles Bibliográficos
Autores principales: Chao, Fa-An, Morelli, Aleardo, Haugner, John C., Churchfield, Lewis, Hagmann, Leonardo N., Shi, Lei, Masterson, Larry R., Sarangi, Ritimukta, Veglia, Gianluigi, Seelig, Burckhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3552148/
https://www.ncbi.nlm.nih.gov/pubmed/23222886
http://dx.doi.org/10.1038/nchembio.1138

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3552148/
https://www.ncbi.nlm.nih.gov/pubmed/23222886
http://dx.doi.org/10.1038/nchembio.1138

Ejemplares similares