Structure and dynamics of a primordial catalytic fold generated by in vitro evolution

Engineering functional protein scaffolds capable of carrying out chemical catalysis is a major challenge in enzyme design. Starting from a non-catalytic protein scaffold, we recently generated a novel RNA ligase by in vitro directed evolution. This artificial enzyme lost its original fold and adopte...

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Autores principales: Chao, Fa-An, Morelli, Aleardo, Haugner, John C., Churchfield, Lewis, Hagmann, Leonardo N., Shi, Lei, Masterson, Larry R., Sarangi, Ritimukta, Veglia, Gianluigi, Seelig, Burckhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3552148/
https://www.ncbi.nlm.nih.gov/pubmed/23222886
http://dx.doi.org/10.1038/nchembio.1138
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author Chao, Fa-An
Morelli, Aleardo
Haugner, John C.
Churchfield, Lewis
Hagmann, Leonardo N.
Shi, Lei
Masterson, Larry R.
Sarangi, Ritimukta
Veglia, Gianluigi
Seelig, Burckhard
author_facet Chao, Fa-An
Morelli, Aleardo
Haugner, John C.
Churchfield, Lewis
Hagmann, Leonardo N.
Shi, Lei
Masterson, Larry R.
Sarangi, Ritimukta
Veglia, Gianluigi
Seelig, Burckhard
author_sort Chao, Fa-An
collection PubMed
description Engineering functional protein scaffolds capable of carrying out chemical catalysis is a major challenge in enzyme design. Starting from a non-catalytic protein scaffold, we recently generated a novel RNA ligase by in vitro directed evolution. This artificial enzyme lost its original fold and adopted an entirely novel structure with dramatically enhanced conformational dynamics, demonstrating that a primordial fold with suitable flexibility is sufficient to carry out enzymatic function.
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spelling pubmed-35521482013-08-01 Structure and dynamics of a primordial catalytic fold generated by in vitro evolution Chao, Fa-An Morelli, Aleardo Haugner, John C. Churchfield, Lewis Hagmann, Leonardo N. Shi, Lei Masterson, Larry R. Sarangi, Ritimukta Veglia, Gianluigi Seelig, Burckhard Nat Chem Biol Article Engineering functional protein scaffolds capable of carrying out chemical catalysis is a major challenge in enzyme design. Starting from a non-catalytic protein scaffold, we recently generated a novel RNA ligase by in vitro directed evolution. This artificial enzyme lost its original fold and adopted an entirely novel structure with dramatically enhanced conformational dynamics, demonstrating that a primordial fold with suitable flexibility is sufficient to carry out enzymatic function. 2012-12-09 2013-02 /pmc/articles/PMC3552148/ /pubmed/23222886 http://dx.doi.org/10.1038/nchembio.1138 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Chao, Fa-An
Morelli, Aleardo
Haugner, John C.
Churchfield, Lewis
Hagmann, Leonardo N.
Shi, Lei
Masterson, Larry R.
Sarangi, Ritimukta
Veglia, Gianluigi
Seelig, Burckhard
Structure and dynamics of a primordial catalytic fold generated by in vitro evolution
title Structure and dynamics of a primordial catalytic fold generated by in vitro evolution
title_full Structure and dynamics of a primordial catalytic fold generated by in vitro evolution
title_fullStr Structure and dynamics of a primordial catalytic fold generated by in vitro evolution
title_full_unstemmed Structure and dynamics of a primordial catalytic fold generated by in vitro evolution
title_short Structure and dynamics of a primordial catalytic fold generated by in vitro evolution
title_sort structure and dynamics of a primordial catalytic fold generated by in vitro evolution
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3552148/
https://www.ncbi.nlm.nih.gov/pubmed/23222886
http://dx.doi.org/10.1038/nchembio.1138
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