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On Measuring miRNAs after Transient Transfection of Mimics or Antisense Inhibitors

The ability to alter microRNA (miRNA) abundance is crucial for studying miRNA function. To achieve this there is widespread use of both exogenous double-stranded miRNA mimics for transient over-expression, and single stranded antisense RNAs (antimiRs) for miRNA inhibition. The success of these manip...

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Detalles Bibliográficos
Autores principales: Thomson, Daniel W., Bracken, Cameron P., Szubert, Jan M., Goodall, Gregory J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3554668/
https://www.ncbi.nlm.nih.gov/pubmed/23358900
http://dx.doi.org/10.1371/journal.pone.0055214