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On Measuring miRNAs after Transient Transfection of Mimics or Antisense Inhibitors
The ability to alter microRNA (miRNA) abundance is crucial for studying miRNA function. To achieve this there is widespread use of both exogenous double-stranded miRNA mimics for transient over-expression, and single stranded antisense RNAs (antimiRs) for miRNA inhibition. The success of these manip...
Autores principales: | Thomson, Daniel W., Bracken, Cameron P., Szubert, Jan M., Goodall, Gregory J. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3554668/ https://www.ncbi.nlm.nih.gov/pubmed/23358900 http://dx.doi.org/10.1371/journal.pone.0055214 |
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