Cargando…

Overexpression of MyrAkt1 in Endothelial Cells Leads to Erythropoietin- and BMP4-Independent Splenic Erythropoiesis in Mice

Under steady state conditions, erythropoiesis occurs in the bone marrow. However, in mice, stress or tissue hypoxia results in increased erythropoiesis in the spleen. There is increasing evidence that the hematopoietic microenvironment, including endothelial cells, plays an important role in regulat...

Descripción completa

Detalles Bibliográficos
Autores principales: O’Donnell, Rebekah K., Goldstein, Whitney E., Perruzzi, Carole, Benjamin, Laura E., Aird, William
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3557261/
https://www.ncbi.nlm.nih.gov/pubmed/23383068
http://dx.doi.org/10.1371/journal.pone.0055095
_version_ 1782257297492279296
author O’Donnell, Rebekah K.
Goldstein, Whitney E.
Perruzzi, Carole
Benjamin, Laura E.
Aird, William
author_facet O’Donnell, Rebekah K.
Goldstein, Whitney E.
Perruzzi, Carole
Benjamin, Laura E.
Aird, William
author_sort O’Donnell, Rebekah K.
collection PubMed
description Under steady state conditions, erythropoiesis occurs in the bone marrow. However, in mice, stress or tissue hypoxia results in increased erythropoiesis in the spleen. There is increasing evidence that the hematopoietic microenvironment, including endothelial cells, plays an important role in regulating erythropoiesis. Here, we show that short-term expression of constitutively active Akt in the endothelium of mice results in non-anemic stress erythropoiesis in the spleen. The initiation of this stress response was independent of erythropoietin and BMP4, and was observed in endothelial myrAkt1 mice reconstituted with wild-type bone marrow. Together, these data suggest that endothelial cell hyperactivation is a potentially novel pathway of inducing red cell production under stress.
format Online
Article
Text
id pubmed-3557261
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-35572612013-02-04 Overexpression of MyrAkt1 in Endothelial Cells Leads to Erythropoietin- and BMP4-Independent Splenic Erythropoiesis in Mice O’Donnell, Rebekah K. Goldstein, Whitney E. Perruzzi, Carole Benjamin, Laura E. Aird, William PLoS One Research Article Under steady state conditions, erythropoiesis occurs in the bone marrow. However, in mice, stress or tissue hypoxia results in increased erythropoiesis in the spleen. There is increasing evidence that the hematopoietic microenvironment, including endothelial cells, plays an important role in regulating erythropoiesis. Here, we show that short-term expression of constitutively active Akt in the endothelium of mice results in non-anemic stress erythropoiesis in the spleen. The initiation of this stress response was independent of erythropoietin and BMP4, and was observed in endothelial myrAkt1 mice reconstituted with wild-type bone marrow. Together, these data suggest that endothelial cell hyperactivation is a potentially novel pathway of inducing red cell production under stress. Public Library of Science 2013-01-28 /pmc/articles/PMC3557261/ /pubmed/23383068 http://dx.doi.org/10.1371/journal.pone.0055095 Text en © 2013 O’Donnell et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
O’Donnell, Rebekah K.
Goldstein, Whitney E.
Perruzzi, Carole
Benjamin, Laura E.
Aird, William
Overexpression of MyrAkt1 in Endothelial Cells Leads to Erythropoietin- and BMP4-Independent Splenic Erythropoiesis in Mice
title Overexpression of MyrAkt1 in Endothelial Cells Leads to Erythropoietin- and BMP4-Independent Splenic Erythropoiesis in Mice
title_full Overexpression of MyrAkt1 in Endothelial Cells Leads to Erythropoietin- and BMP4-Independent Splenic Erythropoiesis in Mice
title_fullStr Overexpression of MyrAkt1 in Endothelial Cells Leads to Erythropoietin- and BMP4-Independent Splenic Erythropoiesis in Mice
title_full_unstemmed Overexpression of MyrAkt1 in Endothelial Cells Leads to Erythropoietin- and BMP4-Independent Splenic Erythropoiesis in Mice
title_short Overexpression of MyrAkt1 in Endothelial Cells Leads to Erythropoietin- and BMP4-Independent Splenic Erythropoiesis in Mice
title_sort overexpression of myrakt1 in endothelial cells leads to erythropoietin- and bmp4-independent splenic erythropoiesis in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3557261/
https://www.ncbi.nlm.nih.gov/pubmed/23383068
http://dx.doi.org/10.1371/journal.pone.0055095
work_keys_str_mv AT odonnellrebekahk overexpressionofmyrakt1inendothelialcellsleadstoerythropoietinandbmp4independentsplenicerythropoiesisinmice
AT goldsteinwhitneye overexpressionofmyrakt1inendothelialcellsleadstoerythropoietinandbmp4independentsplenicerythropoiesisinmice
AT perruzzicarole overexpressionofmyrakt1inendothelialcellsleadstoerythropoietinandbmp4independentsplenicerythropoiesisinmice
AT benjaminlaurae overexpressionofmyrakt1inendothelialcellsleadstoerythropoietinandbmp4independentsplenicerythropoiesisinmice
AT airdwilliam overexpressionofmyrakt1inendothelialcellsleadstoerythropoietinandbmp4independentsplenicerythropoiesisinmice