Chaperone-assisted thermostability engineering of a soluble T cell receptor using phage display

We here report a novel phage display selection strategy enabling fast and easy selection of thermostabilized proteins. The approach is illustrated with stabilization of an aggregation-prone soluble single chain T cell receptor (scTCR) characteristic of the murine MOPC315 myeloma model. Random mutati...

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Detalles Bibliográficos
Autores principales: Gunnarsen, Kristin S., Kristinsson, Solveig G., Justesen, Sune, Frigstad, Terje, Buus, Søren, Bogen, Bjarne, Sandlie, Inger, Løset, Geir Åge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3557450/
https://www.ncbi.nlm.nih.gov/pubmed/23362461
http://dx.doi.org/10.1038/srep01162

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3557450/
https://www.ncbi.nlm.nih.gov/pubmed/23362461
http://dx.doi.org/10.1038/srep01162

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