RNA-programmed genome editing in human cells

Type II CRISPR immune systems in bacteria use a dual RNA-guided DNA endonuclease, Cas9, to cleave foreign DNA at specific sites. We show here that Cas9 assembles with hybrid guide RNAs in human cells and can induce the formation of double-strand DNA breaks (DSBs) at a site complementary to the guide...

Descripción completa

Detalles Bibliográficos
Autores principales: Jinek, Martin, East, Alexandra, Cheng, Aaron, Lin, Steven, Ma, Enbo, Doudna, Jennifer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2013
Materias:
Genomics and Evolutionary Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3557905/
https://www.ncbi.nlm.nih.gov/pubmed/23386978
http://dx.doi.org/10.7554/eLife.00471

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3557905/
https://www.ncbi.nlm.nih.gov/pubmed/23386978
http://dx.doi.org/10.7554/eLife.00471

Ejemplares similares