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Construction of a High Efficiency PCR Products Cloning T Vector Using pGEM-5zf (+)
A highly efficient cloning vector was constructed for cloning PCR products by inserting an 80 bp DNA fragment into pGEM-5zf (+) vector. The Xcm I digestion of this vector gave rise to a 3′ overhanging deoxythymidine offering the possibility of cloning PCR products with 3′ adenosine overhang created...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Avicenna Research Institute
2009
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558116/ https://www.ncbi.nlm.nih.gov/pubmed/23407719 |