A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones

BACKGROUND: Recombineering is a genetic engineering tool that enables facile modification of large episomal clones, e.g. BACs, fosmids. We have previously adapted this technology to generate, directly from fosmid-based genomic clones, fusion gene reporter constructs designed to investigate gene expr...

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Detalles Bibliográficos
Autores principales: Hirani, Nisha, Westenberg, Marcel, Gami, Minaxi S, Davis, Paul, Hope, Ian A, Dolphin, Colin T
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561212/
https://www.ncbi.nlm.nih.gov/pubmed/23281894
http://dx.doi.org/10.1186/1472-6750-13-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561212/
https://www.ncbi.nlm.nih.gov/pubmed/23281894
http://dx.doi.org/10.1186/1472-6750-13-1

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