Vaccinia and influenza A viruses select rather than adjust tRNAs to optimize translation

Transfer RNAs (tRNAs) are central to protein synthesis and impact translational speed and fidelity by their abundance. Here we examine the extent to which viruses manipulate tRNA populations to favor translation of their own genes. We study two very different viruses: influenza A virus (IAV), a medi...

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Detalles Bibliográficos
Autores principales: Pavon-Eternod, Mariana, David, Alexandre, Dittmar, Kimberly, Berglund, Peter, Pan, Tao, Bennink, Jack R., Yewdell, Jonathan W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561966/
https://www.ncbi.nlm.nih.gov/pubmed/23254333
http://dx.doi.org/10.1093/nar/gks986
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author Pavon-Eternod, Mariana
David, Alexandre
Dittmar, Kimberly
Berglund, Peter
Pan, Tao
Bennink, Jack R.
Yewdell, Jonathan W.
author_facet Pavon-Eternod, Mariana
David, Alexandre
Dittmar, Kimberly
Berglund, Peter
Pan, Tao
Bennink, Jack R.
Yewdell, Jonathan W.
author_sort Pavon-Eternod, Mariana
collection PubMed
description Transfer RNAs (tRNAs) are central to protein synthesis and impact translational speed and fidelity by their abundance. Here we examine the extent to which viruses manipulate tRNA populations to favor translation of their own genes. We study two very different viruses: influenza A virus (IAV), a medium-sized (13 kB genome) RNA virus; and vaccinia virus (VV), a large (200 kB genome) DNA virus. We show that the total cellular tRNA population remains unchanged following viral infection, whereas the polysome-associated tRNA population changes dramatically in a virus-specific manner. The changes in polysome-associated tRNA levels reflect the codon usage of viral genes, suggesting the existence of local tRNA pools optimized for viral translation.
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spelling pubmed-35619662013-02-01 Vaccinia and influenza A viruses select rather than adjust tRNAs to optimize translation Pavon-Eternod, Mariana David, Alexandre Dittmar, Kimberly Berglund, Peter Pan, Tao Bennink, Jack R. Yewdell, Jonathan W. Nucleic Acids Res RNA Transfer RNAs (tRNAs) are central to protein synthesis and impact translational speed and fidelity by their abundance. Here we examine the extent to which viruses manipulate tRNA populations to favor translation of their own genes. We study two very different viruses: influenza A virus (IAV), a medium-sized (13 kB genome) RNA virus; and vaccinia virus (VV), a large (200 kB genome) DNA virus. We show that the total cellular tRNA population remains unchanged following viral infection, whereas the polysome-associated tRNA population changes dramatically in a virus-specific manner. The changes in polysome-associated tRNA levels reflect the codon usage of viral genes, suggesting the existence of local tRNA pools optimized for viral translation. Oxford University Press 2013-02 2012-12-18 /pmc/articles/PMC3561966/ /pubmed/23254333 http://dx.doi.org/10.1093/nar/gks986 Text en Published by Oxford University Press 2012. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.
spellingShingle RNA
Pavon-Eternod, Mariana
David, Alexandre
Dittmar, Kimberly
Berglund, Peter
Pan, Tao
Bennink, Jack R.
Yewdell, Jonathan W.
Vaccinia and influenza A viruses select rather than adjust tRNAs to optimize translation
title Vaccinia and influenza A viruses select rather than adjust tRNAs to optimize translation
title_full Vaccinia and influenza A viruses select rather than adjust tRNAs to optimize translation
title_fullStr Vaccinia and influenza A viruses select rather than adjust tRNAs to optimize translation
title_full_unstemmed Vaccinia and influenza A viruses select rather than adjust tRNAs to optimize translation
title_short Vaccinia and influenza A viruses select rather than adjust tRNAs to optimize translation
title_sort vaccinia and influenza a viruses select rather than adjust trnas to optimize translation
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561966/
https://www.ncbi.nlm.nih.gov/pubmed/23254333
http://dx.doi.org/10.1093/nar/gks986
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