AT-rich sequence elements promote nascent transcript cleavage leading to RNA polymerase II termination

RNA Polymerase II (Pol II) termination is dependent on RNA processing signals as well as specific terminator elements located downstream of the poly(A) site. One of the two major terminator classes described so far is the Co-Transcriptional Cleavage (CoTC) element. We show that homopolymer A/T tract...

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Autores principales: White, Eleanor, Kamieniarz-Gdula, Kinga, Dye, Michael J., Proudfoot, Nick J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561976/
https://www.ncbi.nlm.nih.gov/pubmed/23258704
http://dx.doi.org/10.1093/nar/gks1335
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author White, Eleanor
Kamieniarz-Gdula, Kinga
Dye, Michael J.
Proudfoot, Nick J.
author_facet White, Eleanor
Kamieniarz-Gdula, Kinga
Dye, Michael J.
Proudfoot, Nick J.
author_sort White, Eleanor
collection PubMed
description RNA Polymerase II (Pol II) termination is dependent on RNA processing signals as well as specific terminator elements located downstream of the poly(A) site. One of the two major terminator classes described so far is the Co-Transcriptional Cleavage (CoTC) element. We show that homopolymer A/T tracts within the human β-globin CoTC-mediated terminator element play a critical role in Pol II termination. These short A/T tracts, dispersed within seemingly random sequences, are strong terminator elements, and bioinformatics analysis confirms the presence of such sequences in 70% of the putative terminator regions (PTRs) genome-wide.
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spelling pubmed-35619762013-02-01 AT-rich sequence elements promote nascent transcript cleavage leading to RNA polymerase II termination White, Eleanor Kamieniarz-Gdula, Kinga Dye, Michael J. Proudfoot, Nick J. Nucleic Acids Res Molecular Biology RNA Polymerase II (Pol II) termination is dependent on RNA processing signals as well as specific terminator elements located downstream of the poly(A) site. One of the two major terminator classes described so far is the Co-Transcriptional Cleavage (CoTC) element. We show that homopolymer A/T tracts within the human β-globin CoTC-mediated terminator element play a critical role in Pol II termination. These short A/T tracts, dispersed within seemingly random sequences, are strong terminator elements, and bioinformatics analysis confirms the presence of such sequences in 70% of the putative terminator regions (PTRs) genome-wide. Oxford University Press 2013-02 2012-12-19 /pmc/articles/PMC3561976/ /pubmed/23258704 http://dx.doi.org/10.1093/nar/gks1335 Text en © The Author(s) 2012. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Molecular Biology
White, Eleanor
Kamieniarz-Gdula, Kinga
Dye, Michael J.
Proudfoot, Nick J.
AT-rich sequence elements promote nascent transcript cleavage leading to RNA polymerase II termination
title AT-rich sequence elements promote nascent transcript cleavage leading to RNA polymerase II termination
title_full AT-rich sequence elements promote nascent transcript cleavage leading to RNA polymerase II termination
title_fullStr AT-rich sequence elements promote nascent transcript cleavage leading to RNA polymerase II termination
title_full_unstemmed AT-rich sequence elements promote nascent transcript cleavage leading to RNA polymerase II termination
title_short AT-rich sequence elements promote nascent transcript cleavage leading to RNA polymerase II termination
title_sort at-rich sequence elements promote nascent transcript cleavage leading to rna polymerase ii termination
topic Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561976/
https://www.ncbi.nlm.nih.gov/pubmed/23258704
http://dx.doi.org/10.1093/nar/gks1335
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AT dyemichaelj atrichsequenceelementspromotenascenttranscriptcleavageleadingtornapolymeraseiitermination
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