Suppression of artifacts and barcode bias in high-throughput transcriptome analyses utilizing template switching

Template switching (TS) has been an inherent mechanism of reverse transcriptase, which has been exploited in several transcriptome analysis methods, such as CAGE, RNA-Seq and short RNA sequencing. TS is an attractive option, given the simplicity of the protocol, which does not require an adaptor med...

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Detalles Bibliográficos
Autores principales: Tang, Dave T. P., Plessy, Charles, Salimullah, Md, Suzuki, Ana Maria, Calligaris, Raffaella, Gustincich, Stefano, Carninci, Piero
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3562004/
https://www.ncbi.nlm.nih.gov/pubmed/23180801
http://dx.doi.org/10.1093/nar/gks1128

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3562004/
https://www.ncbi.nlm.nih.gov/pubmed/23180801
http://dx.doi.org/10.1093/nar/gks1128

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