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Simple and inexpensive three-step rapid amplification of cDNA 5′ ends using 5′ phosphorylated primers
Rapid amplification of cDNA 5′ ends (5′-RACE) is routinely used for the sequence analysis of the upstream noncoding regions of cellular mRNAs; however, it represents a tedious and cost-intensive procedure. By employing 5′ phosphorylated gene-specific primers for first-strand cDNA synthesis, we cut s...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Academic Press
2013
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3562438/ https://www.ncbi.nlm.nih.gov/pubmed/23123427 http://dx.doi.org/10.1016/j.ab.2012.10.031 |