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A thermoresponsive and chemically defined hydrogel for long-term culture of human embryonic stem cells

Cultures of human embryonic stem cell typically rely on protein matrices or feeder cells to support attachment and growth, while mechanical, enzymatic or chemical cell dissociation methods are used for cellular passaging. However, these methods are ill defined, thus introducing variability into the...

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Detalles Bibliográficos
Autores principales: Zhang, Rong, Mjoseng, Heidi K., Hoeve, Marieke A., Bauer, Nina G., Pells, Steve, Besseling, Rut, Velugotla, Srinivas, Tourniaire, Guilhem, Kishen, Ria E. B., Tsenkina, Yanina, Armit, Chris, Duffy, Cairnan R. E., Helfen, Martina, Edenhofer, Frank, de Sousa, Paul A., Bradley, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3562446/
https://www.ncbi.nlm.nih.gov/pubmed/23299885
http://dx.doi.org/10.1038/ncomms2341