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Time line of redox events in aging postmitotic cells
The precise roles that oxidants play in lifespan and aging are still unknown. Here, we report the discovery that chronologically aging yeast cells undergo a sudden redox collapse, which affects over 80% of identified thiol-containing proteins. We present evidence that this redox collapse is not trig...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3564446/ https://www.ncbi.nlm.nih.gov/pubmed/23390587 http://dx.doi.org/10.7554/eLife.00306 |
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author | Brandes, Nicolas Tienson, Heather Lindemann, Antje Vitvitsky, Victor Reichmann, Dana Banerjee, Ruma Jakob, Ursula |
author_facet | Brandes, Nicolas Tienson, Heather Lindemann, Antje Vitvitsky, Victor Reichmann, Dana Banerjee, Ruma Jakob, Ursula |
author_sort | Brandes, Nicolas |
collection | PubMed |
description | The precise roles that oxidants play in lifespan and aging are still unknown. Here, we report the discovery that chronologically aging yeast cells undergo a sudden redox collapse, which affects over 80% of identified thiol-containing proteins. We present evidence that this redox collapse is not triggered by an increase in endogenous oxidants as would have been postulated by the free radical theory of aging. Instead it appears to be instigated by a substantial drop in cellular NADPH, which normally provides the electron source for maintaining cellular redox homeostasis. This decrease in NADPH levels occurs very early during lifespan and sets into motion a cascade that is predicted to down-regulate most cellular processes. Caloric restriction, a near-universal lifespan extending measure, increases NADPH levels and delays each facet of the cascade. Our studies reveal a time line of events leading up to the system-wide oxidation of the proteome days before cell death. DOI: http://dx.doi.org/10.7554/eLife.00306.001 |
format | Online Article Text |
id | pubmed-3564446 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-35644462013-02-06 Time line of redox events in aging postmitotic cells Brandes, Nicolas Tienson, Heather Lindemann, Antje Vitvitsky, Victor Reichmann, Dana Banerjee, Ruma Jakob, Ursula eLife Cell Biology The precise roles that oxidants play in lifespan and aging are still unknown. Here, we report the discovery that chronologically aging yeast cells undergo a sudden redox collapse, which affects over 80% of identified thiol-containing proteins. We present evidence that this redox collapse is not triggered by an increase in endogenous oxidants as would have been postulated by the free radical theory of aging. Instead it appears to be instigated by a substantial drop in cellular NADPH, which normally provides the electron source for maintaining cellular redox homeostasis. This decrease in NADPH levels occurs very early during lifespan and sets into motion a cascade that is predicted to down-regulate most cellular processes. Caloric restriction, a near-universal lifespan extending measure, increases NADPH levels and delays each facet of the cascade. Our studies reveal a time line of events leading up to the system-wide oxidation of the proteome days before cell death. DOI: http://dx.doi.org/10.7554/eLife.00306.001 eLife Sciences Publications, Ltd 2013-02-05 /pmc/articles/PMC3564446/ /pubmed/23390587 http://dx.doi.org/10.7554/eLife.00306 Text en Copyright © 2013, Brandes et al http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cell Biology Brandes, Nicolas Tienson, Heather Lindemann, Antje Vitvitsky, Victor Reichmann, Dana Banerjee, Ruma Jakob, Ursula Time line of redox events in aging postmitotic cells |
title | Time line of redox events in aging postmitotic cells |
title_full | Time line of redox events in aging postmitotic cells |
title_fullStr | Time line of redox events in aging postmitotic cells |
title_full_unstemmed | Time line of redox events in aging postmitotic cells |
title_short | Time line of redox events in aging postmitotic cells |
title_sort | time line of redox events in aging postmitotic cells |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3564446/ https://www.ncbi.nlm.nih.gov/pubmed/23390587 http://dx.doi.org/10.7554/eLife.00306 |
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