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Nucleosome mobilization by ISW2 requires the concerted action of the ATPase and SLIDE domains
The ISWI family of ATP-dependent chromatin remodelers represses transcription by changing nucleosome positioning. The interactions with extranucleosomal DNA and the requirement of a minimal length of extranucleosomal DNA by ISWI mediate the spacing of nucleosomes. ISW2 from Saccharomyces cerevisiae,...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565048/ https://www.ncbi.nlm.nih.gov/pubmed/23334290 http://dx.doi.org/10.1038/nsmb.2486 |
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author | Hota, Swetansu K. Bhardwaj, Saurabh K. Deindl, Sebastian Lin, Yuan-chi Zhuang, Xiaowei Bartholomew, Blaine |
author_facet | Hota, Swetansu K. Bhardwaj, Saurabh K. Deindl, Sebastian Lin, Yuan-chi Zhuang, Xiaowei Bartholomew, Blaine |
author_sort | Hota, Swetansu K. |
collection | PubMed |
description | The ISWI family of ATP-dependent chromatin remodelers represses transcription by changing nucleosome positioning. The interactions with extranucleosomal DNA and the requirement of a minimal length of extranucleosomal DNA by ISWI mediate the spacing of nucleosomes. ISW2 from Saccharomyces cerevisiae, a member of the ISWI family, has a conserved domain called SLIDE (SANT-like ISWI domain), whose binding to extranucleosomal DNA ~19 bp from the edge of nucleosomes is required for efficiently pushing DNA into nucleosomes and maintaining the unidirectional movement of nucleosomes, as reported here. Loss of SLIDE binding does not perturb ATPase domain binding to the SHL2 site of nucleosomes or its initial movement of DNA inside of nucleosomes. ISW2 has therefore two distinct roles in mobilizing nucleosomes, with the ATPase domain translocating and moving DNA inside nucleosomes, and the SLIDE domain facilitating the entry of linker DNA into nucleosomes. |
format | Online Article Text |
id | pubmed-3565048 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
record_format | MEDLINE/PubMed |
spelling | pubmed-35650482013-08-01 Nucleosome mobilization by ISW2 requires the concerted action of the ATPase and SLIDE domains Hota, Swetansu K. Bhardwaj, Saurabh K. Deindl, Sebastian Lin, Yuan-chi Zhuang, Xiaowei Bartholomew, Blaine Nat Struct Mol Biol Article The ISWI family of ATP-dependent chromatin remodelers represses transcription by changing nucleosome positioning. The interactions with extranucleosomal DNA and the requirement of a minimal length of extranucleosomal DNA by ISWI mediate the spacing of nucleosomes. ISW2 from Saccharomyces cerevisiae, a member of the ISWI family, has a conserved domain called SLIDE (SANT-like ISWI domain), whose binding to extranucleosomal DNA ~19 bp from the edge of nucleosomes is required for efficiently pushing DNA into nucleosomes and maintaining the unidirectional movement of nucleosomes, as reported here. Loss of SLIDE binding does not perturb ATPase domain binding to the SHL2 site of nucleosomes or its initial movement of DNA inside of nucleosomes. ISW2 has therefore two distinct roles in mobilizing nucleosomes, with the ATPase domain translocating and moving DNA inside nucleosomes, and the SLIDE domain facilitating the entry of linker DNA into nucleosomes. 2013-01-20 2013-02 /pmc/articles/PMC3565048/ /pubmed/23334290 http://dx.doi.org/10.1038/nsmb.2486 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Hota, Swetansu K. Bhardwaj, Saurabh K. Deindl, Sebastian Lin, Yuan-chi Zhuang, Xiaowei Bartholomew, Blaine Nucleosome mobilization by ISW2 requires the concerted action of the ATPase and SLIDE domains |
title | Nucleosome mobilization by ISW2 requires the concerted action of the ATPase and SLIDE domains |
title_full | Nucleosome mobilization by ISW2 requires the concerted action of the ATPase and SLIDE domains |
title_fullStr | Nucleosome mobilization by ISW2 requires the concerted action of the ATPase and SLIDE domains |
title_full_unstemmed | Nucleosome mobilization by ISW2 requires the concerted action of the ATPase and SLIDE domains |
title_short | Nucleosome mobilization by ISW2 requires the concerted action of the ATPase and SLIDE domains |
title_sort | nucleosome mobilization by isw2 requires the concerted action of the atpase and slide domains |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565048/ https://www.ncbi.nlm.nih.gov/pubmed/23334290 http://dx.doi.org/10.1038/nsmb.2486 |
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