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A Simple and Robust Vector-Based shRNA Expression System Used for RNA Interference

BACKGROUND: RNA interference (RNAi) mediated by small interfering RNAs (siRNAs) or short hairpin RNAs (shRNAs) has become a powerful genetic tool for conducting functional studies. Previously, vector-based shRNA-expression strategies capable of inducing RNAi in viable cells have been developed, howe...

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Detalles Bibliográficos
Autores principales: Wang, Xue-jun, Li, Ying, Huang, Hai, Zhang, Xiu-juan, Xie, Pei-wen, Hu, Wei, Li, Dan-dan, Wang, Sheng-qi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3566080/
https://www.ncbi.nlm.nih.gov/pubmed/23405258
http://dx.doi.org/10.1371/journal.pone.0056110