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Generation of an Artificial Double Promoter for Protein Expression in Bacillus subtilis through a Promoter Trap System

Bacillus subtilis is an attractive host for production of recombinant proteins. Promoters and expression plasmid backbones have direct impacts on the efficiency of gene expression. To screen and isolate strong promoters, a promoter trap vector pShuttleF was developed in this study. Using the vector,...

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Detalles Bibliográficos
Autores principales: Yang, Mingming, Zhang, Weiwei, Ji, Shengyue, Cao, Pinghua, Chen, Yulin, Zhao, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3568030/
https://www.ncbi.nlm.nih.gov/pubmed/23409173
http://dx.doi.org/10.1371/journal.pone.0056321