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Generation of an Artificial Double Promoter for Protein Expression in Bacillus subtilis through a Promoter Trap System
Bacillus subtilis is an attractive host for production of recombinant proteins. Promoters and expression plasmid backbones have direct impacts on the efficiency of gene expression. To screen and isolate strong promoters, a promoter trap vector pShuttleF was developed in this study. Using the vector,...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3568030/ https://www.ncbi.nlm.nih.gov/pubmed/23409173 http://dx.doi.org/10.1371/journal.pone.0056321 |