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Production of Soluble Human Vascular Endothelial Growth Factor VEGF-A(165)-Heparin Binding Domain in Escherichia coli

We report a method for production of soluble heparin binding domain (HBD) of human vascular endothelial growth factor VEGF-A(165). Recombinant VEGF-A(165)-HBD that contains four disulphide bridges was expressed in specialised E. coli SHuffle cells and its activity has been confirmed through interact...

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Detalles Bibliográficos
Autores principales: Seyedarabi, Arefeh, Cheng, Lili, Zachary, Ian, Djordjevic, Snezana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3568127/
https://www.ncbi.nlm.nih.gov/pubmed/23409021
http://dx.doi.org/10.1371/journal.pone.0055690