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Design and physicochemical characterization of advanced spray-dried tacrolimus multifunctional particles for inhalation

The aim of this study was to design, develop, and optimize respirable tacrolimus microparticles and nanoparticles and multifunctional tacrolimus lung surfactant mimic particles for targeted dry powder inhalation delivery as a pulmonary nanomedicine. Particles were rationally designed and produced at...

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Autores principales: Wu, Xiao, Hayes, Don, Zwischenberger, Joseph B, Kuhn, Robert J, Mansour, Heidi M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3569053/
https://www.ncbi.nlm.nih.gov/pubmed/23403805
http://dx.doi.org/10.2147/DDDT.S40166
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author Wu, Xiao
Hayes, Don
Zwischenberger, Joseph B
Kuhn, Robert J
Mansour, Heidi M
author_facet Wu, Xiao
Hayes, Don
Zwischenberger, Joseph B
Kuhn, Robert J
Mansour, Heidi M
author_sort Wu, Xiao
collection PubMed
description The aim of this study was to design, develop, and optimize respirable tacrolimus microparticles and nanoparticles and multifunctional tacrolimus lung surfactant mimic particles for targeted dry powder inhalation delivery as a pulmonary nanomedicine. Particles were rationally designed and produced at different pump rates by advanced spray-drying particle engineering design from organic solution in closed mode. In addition, multifunctional tacrolimus lung surfactant mimic dry powder particles were prepared by co-dissolving tacrolimus and lung surfactant mimic phospholipids in methanol, followed by advanced co-spray-drying particle engineering design technology in closed mode. The lung surfactant mimic phospholipids were 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and 1,2-dipalmitoyl-sn-glycero-3-[phosphor-rac-1-glycerol]. Laser diffraction particle sizing indicated that the particle size distributions were suitable for pulmonary delivery, whereas scanning electron microscopy imaging indicated that these particles had both optimal particle morphology and surface morphology. Increasing the pump rate percent of tacrolimus solution resulted in a larger particle size. X-ray powder diffraction patterns and differential scanning calorimetry thermograms indicated that spray drying produced particles with higher amounts of amorphous phase. X-ray powder diffraction and differential scanning calorimetry also confirmed the preservation of the phospholipid bilayer structure in the solid state for all engineered respirable particles. Furthermore, it was observed in hot-stage micrographs that raw tacrolimus displayed a liquid crystal transition following the main phase transition, which is consistent with its interfacial properties. Water vapor uptake and lyotropic phase transitions in the solid state at varying levels of relative humidity were determined by gravimetric vapor sorption technique. Water content in the various powders was very low and well within the levels necessary for dry powder inhalation, as quantified by Karl Fisher coulometric titration. Conclusively, advanced spray-drying particle engineering design from organic solution in closed mode was successfully used to design and optimize solid-state particles in the respirable size range necessary for targeted pulmonary delivery, particularly for the deep lung. These particles were dry, stable, and had optimal properties for dry powder inhalation as a novel pulmonary nanomedicine.
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spelling pubmed-35690532013-02-12 Design and physicochemical characterization of advanced spray-dried tacrolimus multifunctional particles for inhalation Wu, Xiao Hayes, Don Zwischenberger, Joseph B Kuhn, Robert J Mansour, Heidi M Drug Des Devel Ther Original Research The aim of this study was to design, develop, and optimize respirable tacrolimus microparticles and nanoparticles and multifunctional tacrolimus lung surfactant mimic particles for targeted dry powder inhalation delivery as a pulmonary nanomedicine. Particles were rationally designed and produced at different pump rates by advanced spray-drying particle engineering design from organic solution in closed mode. In addition, multifunctional tacrolimus lung surfactant mimic dry powder particles were prepared by co-dissolving tacrolimus and lung surfactant mimic phospholipids in methanol, followed by advanced co-spray-drying particle engineering design technology in closed mode. The lung surfactant mimic phospholipids were 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and 1,2-dipalmitoyl-sn-glycero-3-[phosphor-rac-1-glycerol]. Laser diffraction particle sizing indicated that the particle size distributions were suitable for pulmonary delivery, whereas scanning electron microscopy imaging indicated that these particles had both optimal particle morphology and surface morphology. Increasing the pump rate percent of tacrolimus solution resulted in a larger particle size. X-ray powder diffraction patterns and differential scanning calorimetry thermograms indicated that spray drying produced particles with higher amounts of amorphous phase. X-ray powder diffraction and differential scanning calorimetry also confirmed the preservation of the phospholipid bilayer structure in the solid state for all engineered respirable particles. Furthermore, it was observed in hot-stage micrographs that raw tacrolimus displayed a liquid crystal transition following the main phase transition, which is consistent with its interfacial properties. Water vapor uptake and lyotropic phase transitions in the solid state at varying levels of relative humidity were determined by gravimetric vapor sorption technique. Water content in the various powders was very low and well within the levels necessary for dry powder inhalation, as quantified by Karl Fisher coulometric titration. Conclusively, advanced spray-drying particle engineering design from organic solution in closed mode was successfully used to design and optimize solid-state particles in the respirable size range necessary for targeted pulmonary delivery, particularly for the deep lung. These particles were dry, stable, and had optimal properties for dry powder inhalation as a novel pulmonary nanomedicine. Dove Medical Press 2013-02-04 /pmc/articles/PMC3569053/ /pubmed/23403805 http://dx.doi.org/10.2147/DDDT.S40166 Text en © 2013 Wu et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Wu, Xiao
Hayes, Don
Zwischenberger, Joseph B
Kuhn, Robert J
Mansour, Heidi M
Design and physicochemical characterization of advanced spray-dried tacrolimus multifunctional particles for inhalation
title Design and physicochemical characterization of advanced spray-dried tacrolimus multifunctional particles for inhalation
title_full Design and physicochemical characterization of advanced spray-dried tacrolimus multifunctional particles for inhalation
title_fullStr Design and physicochemical characterization of advanced spray-dried tacrolimus multifunctional particles for inhalation
title_full_unstemmed Design and physicochemical characterization of advanced spray-dried tacrolimus multifunctional particles for inhalation
title_short Design and physicochemical characterization of advanced spray-dried tacrolimus multifunctional particles for inhalation
title_sort design and physicochemical characterization of advanced spray-dried tacrolimus multifunctional particles for inhalation
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3569053/
https://www.ncbi.nlm.nih.gov/pubmed/23403805
http://dx.doi.org/10.2147/DDDT.S40166
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