Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification

A cDNA fragment encoding the S-layer protein SllB cloned from Bacillus sphaericus ATCC 14577 was expressed on the surface of E. coli BL21 (DE3) cells and confirmed by the square lattice structure at the nanoscale level. The amplified gene fragment designed with PCR primers from a specified reference...

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Detalles Bibliográficos
Autores principales: CUI, YU-BAO, ZHOU, YING, LIU, WEI-NA, CHEN, QING-WEN, MA, GUI-FANG, SHI, WEI-HONG, WANG, YUN-GANG, YANG, LI
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2012
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3573754/
https://www.ncbi.nlm.nih.gov/pubmed/22266829
http://dx.doi.org/10.3892/ijmm.2012.890

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3573754/
https://www.ncbi.nlm.nih.gov/pubmed/22266829
http://dx.doi.org/10.3892/ijmm.2012.890

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