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Advances in multiplex PCR: balancing primer efficiencies and improving detection success

1. Multiplex PCR is a valuable tool in many biological studies but it is a multifaceted procedure that has to be planned and optimised thoroughly to achieve robust and meaningful results. In particular, primer concentrations have to be adjusted to assure an even amplification of all targeted DNA fra...

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Detalles Bibliográficos
Autores principales: Sint, Daniela, Raso, Lorna, Traugott, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3573865/
https://www.ncbi.nlm.nih.gov/pubmed/23549328
http://dx.doi.org/10.1111/j.2041-210X.2012.00215.x