Cargando…

SecYEG activates GTPases to drive the completion of cotranslational protein targeting

Signal recognition particle (SRP) and its receptor (SR) comprise a highly conserved cellular machine that cotranslationally targets proteins to a protein-conducting channel, the bacterial SecYEG or eukaryotic Sec61p complex, at the target membrane. Whether SecYEG is a passive recipient of the transl...

Descripción completa

Detalles Bibliográficos
Autores principales: Akopian, David, Dalal, Kush, Shen, Kuang, Duong, Franck, Shan, Shu-ou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575545/
https://www.ncbi.nlm.nih.gov/pubmed/23401005
http://dx.doi.org/10.1083/jcb.201208045
_version_ 1782259747456548864
author Akopian, David
Dalal, Kush
Shen, Kuang
Duong, Franck
Shan, Shu-ou
author_facet Akopian, David
Dalal, Kush
Shen, Kuang
Duong, Franck
Shan, Shu-ou
author_sort Akopian, David
collection PubMed
description Signal recognition particle (SRP) and its receptor (SR) comprise a highly conserved cellular machine that cotranslationally targets proteins to a protein-conducting channel, the bacterial SecYEG or eukaryotic Sec61p complex, at the target membrane. Whether SecYEG is a passive recipient of the translating ribosome or actively regulates this targeting machinery remains unclear. Here we show that SecYEG drives conformational changes in the cargo-loaded SRP–SR targeting complex that activate it for GTP hydrolysis and for handover of the translating ribosome. These results provide the first evidence that SecYEG actively drives the efficient delivery and unloading of translating ribosomes at the target membrane.
format Online
Article
Text
id pubmed-3575545
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-35755452013-08-18 SecYEG activates GTPases to drive the completion of cotranslational protein targeting Akopian, David Dalal, Kush Shen, Kuang Duong, Franck Shan, Shu-ou J Cell Biol Research Articles Signal recognition particle (SRP) and its receptor (SR) comprise a highly conserved cellular machine that cotranslationally targets proteins to a protein-conducting channel, the bacterial SecYEG or eukaryotic Sec61p complex, at the target membrane. Whether SecYEG is a passive recipient of the translating ribosome or actively regulates this targeting machinery remains unclear. Here we show that SecYEG drives conformational changes in the cargo-loaded SRP–SR targeting complex that activate it for GTP hydrolysis and for handover of the translating ribosome. These results provide the first evidence that SecYEG actively drives the efficient delivery and unloading of translating ribosomes at the target membrane. The Rockefeller University Press 2013-02-18 /pmc/articles/PMC3575545/ /pubmed/23401005 http://dx.doi.org/10.1083/jcb.201208045 Text en © 2013 Akopian et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Akopian, David
Dalal, Kush
Shen, Kuang
Duong, Franck
Shan, Shu-ou
SecYEG activates GTPases to drive the completion of cotranslational protein targeting
title SecYEG activates GTPases to drive the completion of cotranslational protein targeting
title_full SecYEG activates GTPases to drive the completion of cotranslational protein targeting
title_fullStr SecYEG activates GTPases to drive the completion of cotranslational protein targeting
title_full_unstemmed SecYEG activates GTPases to drive the completion of cotranslational protein targeting
title_short SecYEG activates GTPases to drive the completion of cotranslational protein targeting
title_sort secyeg activates gtpases to drive the completion of cotranslational protein targeting
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575545/
https://www.ncbi.nlm.nih.gov/pubmed/23401005
http://dx.doi.org/10.1083/jcb.201208045
work_keys_str_mv AT akopiandavid secyegactivatesgtpasestodrivethecompletionofcotranslationalproteintargeting
AT dalalkush secyegactivatesgtpasestodrivethecompletionofcotranslationalproteintargeting
AT shenkuang secyegactivatesgtpasestodrivethecompletionofcotranslationalproteintargeting
AT duongfranck secyegactivatesgtpasestodrivethecompletionofcotranslationalproteintargeting
AT shanshuou secyegactivatesgtpasestodrivethecompletionofcotranslationalproteintargeting