Synthetic gene expression perturbation systems with rapid, tunable, single-gene specificity in yeast

A general method for the dynamic control of single gene expression in eukaryotes, with no off-target effects, is a long-sought tool for molecular and systems biologists. We engineered two artificial transcription factors (ATFs) that contain Cys(2)His(2) zinc-finger DNA-binding domains of either the...

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Detalles Bibliográficos
Autores principales: McIsaac, R. Scott, Oakes, Benjamin L., Wang, Xin, Dummit, Krysta A., Botstein, David, Noyes, Marcus B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575806/
https://www.ncbi.nlm.nih.gov/pubmed/23275543
http://dx.doi.org/10.1093/nar/gks1313

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575806/
https://www.ncbi.nlm.nih.gov/pubmed/23275543
http://dx.doi.org/10.1093/nar/gks1313

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