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50nm-Scale Localization of Single Unmodified, Isotopically Enriched, Proteins in Cells

Imaging single proteins within cells is challenging if the possibility of artefacts due to tagging or to recognition by antibodies is to be avoided. It is generally believed that the biological properties of proteins remain unaltered when (14)N isotopes are replaced with (15)N. (15)N-enriched protei...

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Detalles Bibliográficos
Autores principales: Delaune, Anthony, Cabin-Flaman, Armelle, Legent, Guillaume, Gibouin, David, Smet-Nocca, Caroline, Lefebvre, Fabrice, Benecke, Arndt, Vasse, Marc, Ripoll, Camille
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3576336/
https://www.ncbi.nlm.nih.gov/pubmed/23431383
http://dx.doi.org/10.1371/journal.pone.0056559