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Expression and Purification of Functional Human Mu Opioid Receptor from E.coli

N-terminally his-tagged human mu opioid receptor, a G protein-coupled receptor was produced in E.coli employing synthetic codon-usage optimized constructs. The receptor was expressed in inclusion bodies and membrane-inserted in different E.coli strains. By optimizing the expression conditions the ex...

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Detalles Bibliográficos
Autores principales: Ma, Yanbin, Kubicek, Jan, Labahn, Jörg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3578875/
https://www.ncbi.nlm.nih.gov/pubmed/23437147
http://dx.doi.org/10.1371/journal.pone.0056500