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Artificial Environments for the Co-Translational Stabilization of Cell-Free Expressed Proteins
An approach for designing individual expression environments that reduce or prevent protein aggregation and precipitation is described. Inefficient folding of difficult proteins in unfavorable translation environments can cause significant losses of overexpressed proteins as precipitates or inclusio...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3579822/ https://www.ncbi.nlm.nih.gov/pubmed/23451062 http://dx.doi.org/10.1371/journal.pone.0056637 |