Cargando…
Artificial Environments for the Co-Translational Stabilization of Cell-Free Expressed Proteins
An approach for designing individual expression environments that reduce or prevent protein aggregation and precipitation is described. Inefficient folding of difficult proteins in unfavorable translation environments can cause significant losses of overexpressed proteins as precipitates or inclusio...
Autores principales: | Kai, Lei, Dötsch, Volker, Kaldenhoff, Ralf, Bernhard, Frank |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3579822/ https://www.ncbi.nlm.nih.gov/pubmed/23451062 http://dx.doi.org/10.1371/journal.pone.0056637 |
Ejemplares similares
-
Preparative Scale Production of Functional Mouse Aquaporin 4 Using Different Cell-Free Expression Modes
por: Kai, Lei, et al.
Publicado: (2010) -
Co-Translational Insertion of Aquaporins into Liposome for Functional Analysis via an E. coli Based Cell-Free Protein Synthesis System
por: Yue, Ke, et al.
Publicado: (2019) -
A refined model of water and CO(2) membrane diffusion: Effects and contribution of sterols and proteins
por: Kai, Lei, et al.
Publicado: (2014) -
Transfer mechanism of cell-free synthesized membrane proteins into mammalian cells
por: Umbach, Simon, et al.
Publicado: (2022) -
E. coli “Stablelabel” S30 lysate for optimized cell-free NMR sample preparation
por: Levin, Roman, et al.
Publicado: (2023)