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Artificial Environments for the Co-Translational Stabilization of Cell-Free Expressed Proteins

An approach for designing individual expression environments that reduce or prevent protein aggregation and precipitation is described. Inefficient folding of difficult proteins in unfavorable translation environments can cause significant losses of overexpressed proteins as precipitates or inclusio...

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Detalles Bibliográficos
Autores principales: Kai, Lei, Dötsch, Volker, Kaldenhoff, Ralf, Bernhard, Frank
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3579822/
https://www.ncbi.nlm.nih.gov/pubmed/23451062
http://dx.doi.org/10.1371/journal.pone.0056637

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