Efficient E. coli Expression Strategies for Production of Soluble Human Crystallin ALDH3A1

Aldehyde dehydrogenase 3A1 (ALDH3A1) is a recently characterized corneal crystallin with its exact functions still being unclear. Expressing recombinant human ALDH3A1 has been difficult in Escherichia coli (E. coli) because of low solubility, yield and insufficient purity issues. In this report, we...

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Detalles Bibliográficos
Autores principales: Voulgaridou, Georgia-Persephoni, Mantso, Theodora, Chlichlia, Katerina, Panayiotidis, Mihalis I., Pappa, Aglaia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3579847/
https://www.ncbi.nlm.nih.gov/pubmed/23451057
http://dx.doi.org/10.1371/journal.pone.0056582

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3579847/
https://www.ncbi.nlm.nih.gov/pubmed/23451057
http://dx.doi.org/10.1371/journal.pone.0056582

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