Directly Transforming PCR-Amplified DNA Fragments into Plant Cells Is a Versatile System That Facilitates the Transient Expression Assay

A circular plasmid containing a gene coding sequence has been broadly used for studying gene regulation in cells. However, to accommodate a quick screen plasmid construction and preparation can be time consuming. Here we report a PCR amplified dsDNA fragments (PCR-fragments) based transient expressi...

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Detalles Bibliográficos
Autores principales: Lu, Yuming, Chen, Xi, Wu, Yuxuan, Wang, Yanping, He, Yuqing, Wu, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3582559/
https://www.ncbi.nlm.nih.gov/pubmed/23468926
http://dx.doi.org/10.1371/journal.pone.0057171

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3582559/
https://www.ncbi.nlm.nih.gov/pubmed/23468926
http://dx.doi.org/10.1371/journal.pone.0057171

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