Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus

hTERTC27, a 27-kDa hTERT C-terminal polypeptide has been demonstrated to cause hTERT-positive HeLa cell apoptosis and inhibits the growth of mouse melanoma. hTERTC27 has been associated with telomere dysfunction, regulation of gene-regulated apoptosis, the cell cycle and activation of natural killer...

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Autores principales: GONG, HAN-XIAN, HE, LEI, LI, XIANG-PEN, WANG, YI-DONG, LI, YI, HUANG, JUN-JIAN, WANG, ZILING, XIE, DAN, KUNG, HSIANG-FU, PENG, YING
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3583520/
https://www.ncbi.nlm.nih.gov/pubmed/22210010
http://dx.doi.org/10.3892/or.2011.1619
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author GONG, HAN-XIAN
HE, LEI
LI, XIANG-PEN
WANG, YI-DONG
LI, YI
HUANG, JUN-JIAN
WANG, ZILING
XIE, DAN
KUNG, HSIANG-FU
PENG, YING
author_facet GONG, HAN-XIAN
HE, LEI
LI, XIANG-PEN
WANG, YI-DONG
LI, YI
HUANG, JUN-JIAN
WANG, ZILING
XIE, DAN
KUNG, HSIANG-FU
PENG, YING
author_sort GONG, HAN-XIAN
collection PubMed
description hTERTC27, a 27-kDa hTERT C-terminal polypeptide has been demonstrated to cause hTERT-positive HeLa cell apoptosis and inhibits the growth of mouse melanoma. hTERTC27 has been associated with telomere dysfunction, regulation of gene-regulated apoptosis, the cell cycle and activation of natural killer (NK) cells, but its mechanism of action is not fully understood. Here, we report that dendritic cells (DCs) transduced with hTERTC27 can increase T-cell proliferation, and augment the concentration of interleukin-2 (IL-2) and interferon-γ (IFN-γ) in the supernatants of T cells. It can also induce antigen-specific cytotoxic T lymphocytes (CTL) against glioma cells in vitro. Moreover, hTERTC27 gene-transduced DCs exhibit a very potent cytotoxicity to glioma cells in vivo. It could prolong the survival time and inhibit the growth of glioma-bearing mice. These data suggest that hTERTC27 gene-transduced DCs can efficiently enhance immunity against gliomas in vitro and in vivo.
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spelling pubmed-35835202013-02-28 Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus GONG, HAN-XIAN HE, LEI LI, XIANG-PEN WANG, YI-DONG LI, YI HUANG, JUN-JIAN WANG, ZILING XIE, DAN KUNG, HSIANG-FU PENG, YING Oncol Rep Articles hTERTC27, a 27-kDa hTERT C-terminal polypeptide has been demonstrated to cause hTERT-positive HeLa cell apoptosis and inhibits the growth of mouse melanoma. hTERTC27 has been associated with telomere dysfunction, regulation of gene-regulated apoptosis, the cell cycle and activation of natural killer (NK) cells, but its mechanism of action is not fully understood. Here, we report that dendritic cells (DCs) transduced with hTERTC27 can increase T-cell proliferation, and augment the concentration of interleukin-2 (IL-2) and interferon-γ (IFN-γ) in the supernatants of T cells. It can also induce antigen-specific cytotoxic T lymphocytes (CTL) against glioma cells in vitro. Moreover, hTERTC27 gene-transduced DCs exhibit a very potent cytotoxicity to glioma cells in vivo. It could prolong the survival time and inhibit the growth of glioma-bearing mice. These data suggest that hTERTC27 gene-transduced DCs can efficiently enhance immunity against gliomas in vitro and in vivo. D.A. Spandidos 2011-12-30 2012-04 /pmc/articles/PMC3583520/ /pubmed/22210010 http://dx.doi.org/10.3892/or.2011.1619 Text en Copyright © 2012, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
GONG, HAN-XIAN
HE, LEI
LI, XIANG-PEN
WANG, YI-DONG
LI, YI
HUANG, JUN-JIAN
WANG, ZILING
XIE, DAN
KUNG, HSIANG-FU
PENG, YING
Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus
title Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus
title_full Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus
title_fullStr Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus
title_full_unstemmed Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus
title_short Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus
title_sort effective antitumor immunity against murine gliomas using dendritic cells transduced with htertc27 recombinant adenovirus
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3583520/
https://www.ncbi.nlm.nih.gov/pubmed/22210010
http://dx.doi.org/10.3892/or.2011.1619
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