Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus
hTERTC27, a 27-kDa hTERT C-terminal polypeptide has been demonstrated to cause hTERT-positive HeLa cell apoptosis and inhibits the growth of mouse melanoma. hTERTC27 has been associated with telomere dysfunction, regulation of gene-regulated apoptosis, the cell cycle and activation of natural killer...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3583520/ https://www.ncbi.nlm.nih.gov/pubmed/22210010 http://dx.doi.org/10.3892/or.2011.1619 |
_version_ | 1782475435797381120 |
---|---|
author | GONG, HAN-XIAN HE, LEI LI, XIANG-PEN WANG, YI-DONG LI, YI HUANG, JUN-JIAN WANG, ZILING XIE, DAN KUNG, HSIANG-FU PENG, YING |
author_facet | GONG, HAN-XIAN HE, LEI LI, XIANG-PEN WANG, YI-DONG LI, YI HUANG, JUN-JIAN WANG, ZILING XIE, DAN KUNG, HSIANG-FU PENG, YING |
author_sort | GONG, HAN-XIAN |
collection | PubMed |
description | hTERTC27, a 27-kDa hTERT C-terminal polypeptide has been demonstrated to cause hTERT-positive HeLa cell apoptosis and inhibits the growth of mouse melanoma. hTERTC27 has been associated with telomere dysfunction, regulation of gene-regulated apoptosis, the cell cycle and activation of natural killer (NK) cells, but its mechanism of action is not fully understood. Here, we report that dendritic cells (DCs) transduced with hTERTC27 can increase T-cell proliferation, and augment the concentration of interleukin-2 (IL-2) and interferon-γ (IFN-γ) in the supernatants of T cells. It can also induce antigen-specific cytotoxic T lymphocytes (CTL) against glioma cells in vitro. Moreover, hTERTC27 gene-transduced DCs exhibit a very potent cytotoxicity to glioma cells in vivo. It could prolong the survival time and inhibit the growth of glioma-bearing mice. These data suggest that hTERTC27 gene-transduced DCs can efficiently enhance immunity against gliomas in vitro and in vivo. |
format | Online Article Text |
id | pubmed-3583520 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-35835202013-02-28 Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus GONG, HAN-XIAN HE, LEI LI, XIANG-PEN WANG, YI-DONG LI, YI HUANG, JUN-JIAN WANG, ZILING XIE, DAN KUNG, HSIANG-FU PENG, YING Oncol Rep Articles hTERTC27, a 27-kDa hTERT C-terminal polypeptide has been demonstrated to cause hTERT-positive HeLa cell apoptosis and inhibits the growth of mouse melanoma. hTERTC27 has been associated with telomere dysfunction, regulation of gene-regulated apoptosis, the cell cycle and activation of natural killer (NK) cells, but its mechanism of action is not fully understood. Here, we report that dendritic cells (DCs) transduced with hTERTC27 can increase T-cell proliferation, and augment the concentration of interleukin-2 (IL-2) and interferon-γ (IFN-γ) in the supernatants of T cells. It can also induce antigen-specific cytotoxic T lymphocytes (CTL) against glioma cells in vitro. Moreover, hTERTC27 gene-transduced DCs exhibit a very potent cytotoxicity to glioma cells in vivo. It could prolong the survival time and inhibit the growth of glioma-bearing mice. These data suggest that hTERTC27 gene-transduced DCs can efficiently enhance immunity against gliomas in vitro and in vivo. D.A. Spandidos 2011-12-30 2012-04 /pmc/articles/PMC3583520/ /pubmed/22210010 http://dx.doi.org/10.3892/or.2011.1619 Text en Copyright © 2012, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles GONG, HAN-XIAN HE, LEI LI, XIANG-PEN WANG, YI-DONG LI, YI HUANG, JUN-JIAN WANG, ZILING XIE, DAN KUNG, HSIANG-FU PENG, YING Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus |
title | Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus |
title_full | Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus |
title_fullStr | Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus |
title_full_unstemmed | Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus |
title_short | Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus |
title_sort | effective antitumor immunity against murine gliomas using dendritic cells transduced with htertc27 recombinant adenovirus |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3583520/ https://www.ncbi.nlm.nih.gov/pubmed/22210010 http://dx.doi.org/10.3892/or.2011.1619 |
work_keys_str_mv | AT gonghanxian effectiveantitumorimmunityagainstmurinegliomasusingdendriticcellstransducedwithhtertc27recombinantadenovirus AT helei effectiveantitumorimmunityagainstmurinegliomasusingdendriticcellstransducedwithhtertc27recombinantadenovirus AT lixiangpen effectiveantitumorimmunityagainstmurinegliomasusingdendriticcellstransducedwithhtertc27recombinantadenovirus AT wangyidong effectiveantitumorimmunityagainstmurinegliomasusingdendriticcellstransducedwithhtertc27recombinantadenovirus AT liyi effectiveantitumorimmunityagainstmurinegliomasusingdendriticcellstransducedwithhtertc27recombinantadenovirus AT huangjunjian effectiveantitumorimmunityagainstmurinegliomasusingdendriticcellstransducedwithhtertc27recombinantadenovirus AT wangziling effectiveantitumorimmunityagainstmurinegliomasusingdendriticcellstransducedwithhtertc27recombinantadenovirus AT xiedan effectiveantitumorimmunityagainstmurinegliomasusingdendriticcellstransducedwithhtertc27recombinantadenovirus AT kunghsiangfu effectiveantitumorimmunityagainstmurinegliomasusingdendriticcellstransducedwithhtertc27recombinantadenovirus AT pengying effectiveantitumorimmunityagainstmurinegliomasusingdendriticcellstransducedwithhtertc27recombinantadenovirus |