Robust, synergistic regulation of human gene expression using TALE activators

Artificial transcription activator-like (TAL) effector-based activators (TALE activators) have broad utility but previous studies suggest that these monomeric proteins often possess low activities. Here we demonstrate that TALE activators can robustly function individually or in synergistic combinat...

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Autores principales: Maeder, Morgan L., Linder, Samantha J., Reyon, Deepak, Angstman, James F., Fu, Yanfang, Sander, Jeffry D., Joung, J. Keith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584229/
https://www.ncbi.nlm.nih.gov/pubmed/23396285
http://dx.doi.org/10.1038/nmeth.2366
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author Maeder, Morgan L.
Linder, Samantha J.
Reyon, Deepak
Angstman, James F.
Fu, Yanfang
Sander, Jeffry D.
Joung, J. Keith
author_facet Maeder, Morgan L.
Linder, Samantha J.
Reyon, Deepak
Angstman, James F.
Fu, Yanfang
Sander, Jeffry D.
Joung, J. Keith
author_sort Maeder, Morgan L.
collection PubMed
description Artificial transcription activator-like (TAL) effector-based activators (TALE activators) have broad utility but previous studies suggest that these monomeric proteins often possess low activities. Here we demonstrate that TALE activators can robustly function individually or in synergistic combinations to increase expression of endogenous human genes over wide dynamic ranges. These findings will encourage applications of TALE activators for research and therapy and guide design of novel monomeric TAL effector-based fusion proteins.
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spelling pubmed-35842292013-09-01 Robust, synergistic regulation of human gene expression using TALE activators Maeder, Morgan L. Linder, Samantha J. Reyon, Deepak Angstman, James F. Fu, Yanfang Sander, Jeffry D. Joung, J. Keith Nat Methods Article Artificial transcription activator-like (TAL) effector-based activators (TALE activators) have broad utility but previous studies suggest that these monomeric proteins often possess low activities. Here we demonstrate that TALE activators can robustly function individually or in synergistic combinations to increase expression of endogenous human genes over wide dynamic ranges. These findings will encourage applications of TALE activators for research and therapy and guide design of novel monomeric TAL effector-based fusion proteins. 2013-02-10 2013-03 /pmc/articles/PMC3584229/ /pubmed/23396285 http://dx.doi.org/10.1038/nmeth.2366 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Maeder, Morgan L.
Linder, Samantha J.
Reyon, Deepak
Angstman, James F.
Fu, Yanfang
Sander, Jeffry D.
Joung, J. Keith
Robust, synergistic regulation of human gene expression using TALE activators
title Robust, synergistic regulation of human gene expression using TALE activators
title_full Robust, synergistic regulation of human gene expression using TALE activators
title_fullStr Robust, synergistic regulation of human gene expression using TALE activators
title_full_unstemmed Robust, synergistic regulation of human gene expression using TALE activators
title_short Robust, synergistic regulation of human gene expression using TALE activators
title_sort robust, synergistic regulation of human gene expression using tale activators
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584229/
https://www.ncbi.nlm.nih.gov/pubmed/23396285
http://dx.doi.org/10.1038/nmeth.2366
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