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Faster Protein Splicing with the Nostoc punctiforme DnaE Intein Using Non-native Extein Residues

Inteins are naturally occurring intervening sequences that catalyze a protein splicing reaction resulting in intein excision and concatenation of the flanking polypeptides (exteins) with a native peptide bond. Inteins display a diversity of catalytic mechanisms within a highly conserved fold that is...

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Detalles Bibliográficos
Autores principales: Cheriyan, Manoj, Pedamallu, Chandra Sekhar, Tori, Kazuo, Perler, Francine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3585056/
https://www.ncbi.nlm.nih.gov/pubmed/23306197
http://dx.doi.org/10.1074/jbc.M112.433094