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Decreased Dicer Expression Enhances SRP-Mediated Protein Targeting

We have shown that Dicer processes 7SL RNA into different fragments ranging from ∼20 to more than 200 nucleotides. Here we addressed the molecular functions of these 7SL RNA fragments and found that some of them functioned as dominant-negative regulators of the full-length 7SL RNA, interfering with...

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Detalles Bibliográficos
Autores principales: Ren, Yong-Feng, Li, Guiling, Xue, Yong-Feng, Zhang, Xue-Jiao, Song, Yi-Jiang, Lv, Lu, Wu, Jianmin, Fang, Yu-Xiao, Wang, Yu-Qun, Shi, Ke-Qing, Chen, Yong-ping, Tang, Kai-Fu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3585229/
https://www.ncbi.nlm.nih.gov/pubmed/23468895
http://dx.doi.org/10.1371/journal.pone.0056950
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author Ren, Yong-Feng
Li, Guiling
Xue, Yong-Feng
Zhang, Xue-Jiao
Song, Yi-Jiang
Lv, Lu
Wu, Jianmin
Fang, Yu-Xiao
Wang, Yu-Qun
Shi, Ke-Qing
Chen, Yong-ping
Tang, Kai-Fu
author_facet Ren, Yong-Feng
Li, Guiling
Xue, Yong-Feng
Zhang, Xue-Jiao
Song, Yi-Jiang
Lv, Lu
Wu, Jianmin
Fang, Yu-Xiao
Wang, Yu-Qun
Shi, Ke-Qing
Chen, Yong-ping
Tang, Kai-Fu
author_sort Ren, Yong-Feng
collection PubMed
description We have shown that Dicer processes 7SL RNA into different fragments ranging from ∼20 to more than 200 nucleotides. Here we addressed the molecular functions of these 7SL RNA fragments and found that some of them functioned as dominant-negative regulators of the full-length 7SL RNA, interfering with signal recognition particle (SRP) complex formation. Transfection of these 7SL RNA fragments inhibited the expression of cell surface glycoproteins, the targeting of a reporter protein to the endoplasmic reticulum, and the secretion of secreted alkaline phosphatase. These results suggest that some Dicer-processed 7SL RNA fragments interfered with SRP-mediated protein targeting. Moreover, we showed that Dicer knockdown enhanced SRP-mediated protein targeting and that transfection of a mixture of the 7SL RNA fragments partially restored this effect. Our data indicate that Dicer can fine-tune the efficiency of SRP-mediated protein targeting via processing a proportion of 7SL RNA into fragments of different lengths.
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spelling pubmed-35852292013-03-06 Decreased Dicer Expression Enhances SRP-Mediated Protein Targeting Ren, Yong-Feng Li, Guiling Xue, Yong-Feng Zhang, Xue-Jiao Song, Yi-Jiang Lv, Lu Wu, Jianmin Fang, Yu-Xiao Wang, Yu-Qun Shi, Ke-Qing Chen, Yong-ping Tang, Kai-Fu PLoS One Research Article We have shown that Dicer processes 7SL RNA into different fragments ranging from ∼20 to more than 200 nucleotides. Here we addressed the molecular functions of these 7SL RNA fragments and found that some of them functioned as dominant-negative regulators of the full-length 7SL RNA, interfering with signal recognition particle (SRP) complex formation. Transfection of these 7SL RNA fragments inhibited the expression of cell surface glycoproteins, the targeting of a reporter protein to the endoplasmic reticulum, and the secretion of secreted alkaline phosphatase. These results suggest that some Dicer-processed 7SL RNA fragments interfered with SRP-mediated protein targeting. Moreover, we showed that Dicer knockdown enhanced SRP-mediated protein targeting and that transfection of a mixture of the 7SL RNA fragments partially restored this effect. Our data indicate that Dicer can fine-tune the efficiency of SRP-mediated protein targeting via processing a proportion of 7SL RNA into fragments of different lengths. Public Library of Science 2013-02-28 /pmc/articles/PMC3585229/ /pubmed/23468895 http://dx.doi.org/10.1371/journal.pone.0056950 Text en © 2013 Ren et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ren, Yong-Feng
Li, Guiling
Xue, Yong-Feng
Zhang, Xue-Jiao
Song, Yi-Jiang
Lv, Lu
Wu, Jianmin
Fang, Yu-Xiao
Wang, Yu-Qun
Shi, Ke-Qing
Chen, Yong-ping
Tang, Kai-Fu
Decreased Dicer Expression Enhances SRP-Mediated Protein Targeting
title Decreased Dicer Expression Enhances SRP-Mediated Protein Targeting
title_full Decreased Dicer Expression Enhances SRP-Mediated Protein Targeting
title_fullStr Decreased Dicer Expression Enhances SRP-Mediated Protein Targeting
title_full_unstemmed Decreased Dicer Expression Enhances SRP-Mediated Protein Targeting
title_short Decreased Dicer Expression Enhances SRP-Mediated Protein Targeting
title_sort decreased dicer expression enhances srp-mediated protein targeting
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3585229/
https://www.ncbi.nlm.nih.gov/pubmed/23468895
http://dx.doi.org/10.1371/journal.pone.0056950
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