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Correlative In Vivo 2 Photon and Focused Ion Beam Scanning Electron Microscopy of Cortical Neurons

Correlating in vivo imaging of neurons and their synaptic connections with electron microscopy combines dynamic and ultrastructural information. Here we describe a semi-automated technique whereby volumes of brain tissue containing axons and dendrites, previously studied in vivo, are subsequently im...

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Detalles Bibliográficos
Autores principales: Maco, Bohumil, Holtmaat, Anthony, Cantoni, Marco, Kreshuk, Anna, Straehle, Christoph N., Hamprecht, Fred A., Knott, Graham W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3585404/
https://www.ncbi.nlm.nih.gov/pubmed/23468982
http://dx.doi.org/10.1371/journal.pone.0057405