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Correlative In Vivo 2 Photon and Focused Ion Beam Scanning Electron Microscopy of Cortical Neurons
Correlating in vivo imaging of neurons and their synaptic connections with electron microscopy combines dynamic and ultrastructural information. Here we describe a semi-automated technique whereby volumes of brain tissue containing axons and dendrites, previously studied in vivo, are subsequently im...
Autores principales: | Maco, Bohumil, Holtmaat, Anthony, Cantoni, Marco, Kreshuk, Anna, Straehle, Christoph N., Hamprecht, Fred A., Knott, Graham W. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3585404/ https://www.ncbi.nlm.nih.gov/pubmed/23468982 http://dx.doi.org/10.1371/journal.pone.0057405 |
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