2-(2-Hydroxy-5-nitrobenzylidene)-1,3-indanedione versus Fluorescein Isothiocyanate in Interaction with Anti-hFABP Immunoglobulin G1: Fluorescence Quenching, Secondary Structure Alteration and Binding Sites Localization

The first step in determining whether a fluorescent dye can be used for antibody labeling consists in collecting data on its physical interaction with the latter. In the present study, the interaction between the 2-(2-hydroxy-5-nitrobenzylidene)-1,3-indanedione (HNBID) dye and the IgG1 monoclonal mo...

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Detalles Bibliográficos
Autores principales: Stan, Dana, Mihailescu, Carmen-Marinela, Savin, Mihaela, Matei, Iulia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3588028/
https://www.ncbi.nlm.nih.gov/pubmed/23434655
http://dx.doi.org/10.3390/ijms14023011

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3588028/
https://www.ncbi.nlm.nih.gov/pubmed/23434655
http://dx.doi.org/10.3390/ijms14023011

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