Cargando…

2-(2-Hydroxy-5-nitrobenzylidene)-1,3-indanedione versus Fluorescein Isothiocyanate in Interaction with Anti-hFABP Immunoglobulin G1: Fluorescence Quenching, Secondary Structure Alteration and Binding Sites Localization

The first step in determining whether a fluorescent dye can be used for antibody labeling consists in collecting data on its physical interaction with the latter. In the present study, the interaction between the 2-(2-hydroxy-5-nitrobenzylidene)-1,3-indanedione (HNBID) dye and the IgG1 monoclonal mo...

Descripción completa

Detalles Bibliográficos
Autores principales:	Stan, Dana, Mihailescu, Carmen-Marinela, Savin, Mihaela, Matei, Iulia
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2013
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3588028/ https://www.ncbi.nlm.nih.gov/pubmed/23434655 http://dx.doi.org/10.3390/ijms14023011

Ejemplares similares

Spectroscopic Investigations of the Binding Interaction of a New Indanedione Derivative with Human and Bovine Serum Albumins
por: Stan, Dana, et al.
Publicado: (2009)

Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma
por: Ogawa, Takehito, et al.
Publicado: (2009)

Honey-Induced Protein Stabilization as Studied by Fluorescein Isothiocyanate Fluorescence
por: Wong, Yin How, et al.
Publicado: (2013)

Ischemic biomarker heart-type fatty acid binding protein (hFABP) in acute heart failure - diagnostic and prognostic insights compared to NT-proBNP and troponin I
por: Hoffmann, Ursula, et al.
Publicado: (2015)

4-Hydroxy-N′-(4-hydroxy-3-nitrobenzylidene)benzohydrazide
por: Zhang, Zhen
Publicado: (2011)

2-Hydroxy-N′-(4-hydroxy-3-nitrobenzylidene)benzohydrazide
por: Zhang, Zhen
Publicado: (2011)

4-Hydroxy-N′-(3-nitrobenzylidene)benzohydrazide
por: Hou, Jin-Long
Publicado: (2012)

3-Hydroxy-N′-(5-hydroxy-2-nitrobenzylidene)-2-naphthohydrazide
por: Yang, De-Suo
Publicado: (2009)

Comparison of the Photobleaching and Photostability Traits of Alexa Fluor 568- and Fluorescein Isothiocyanate- conjugated Antibody
por: Mahmoudian, Jafar, et al.
Publicado: (2011)

N′-(2-Hydroxy-5-nitrobenzylidene)benzenesulfonohydrazide
por: Yusnita, Juahir, et al.
Publicado: (2008)

(E)-4-Hydroxy-N′-(4-nitrobenzylidene)benzohydrazide
por: Li, Cong-Ming, et al.
Publicado: (2009)

2-Hydroxy-N′-(5-hydroxy-2-nitrobenzylidene)-3-methylbenzohydrazide
por: Zhu, Zhao-Fu, et al.
Publicado: (2012)

Synthesis and screening of cyclic diketone indanedione derivatives as future scaffolds for neutrophil elastase inhibition
por: S., Meena, et al.
Publicado: (2023)

Assay considerations for fluorescein isothiocyanate-dextran (FITC-d): an indicator of intestinal permeability in broiler chickens
por: Liu, Jundi, et al.
Publicado: (2021)

4-[(2-Hydroxy-5-nitrobenzylidene)amino]benzenesulfonamide
por: Tan, Yi-Han, et al.
Publicado: (2010)

2-Chloro-N′-(5-hydroxy-2-nitrobenzylidene)benzohydrazide
por: Zhou, Cong-Shan, et al.
Publicado: (2010)

N′-(5-Hydroxy-2-nitrobenzylidene)-2-methoxybenzohydrazide
por: Yang, De-Suo
Publicado: (2009)

4-[1-(Hydroxyimino)ethyl]-N-(4-nitrobenzylidene)aniline
por: Zhao, Li, et al.
Publicado: (2009)

Determination of Trace Deoxyribonucleic Acid by Using Fluorescein Isothiocyanate-Phenosafranine as a Double-Luminescent Phosphorescence Probe
por: Liu, Jia-Ming, et al.
Publicado: (2010)

Increased Intratumor Concentration of Fluorescein‐isothiocyanate‐labeled Neocarzino‐statin in Rats under Angiotensin‐induced Hypertension
por: Abe, Ikuo, et al.
Publicado: (1988)

2-Cyano-N′-(5-hydroxy-2-nitrobenzylidene)acetohydrazide monohydrate
por: Li, Hongbo, et al.
Publicado: (2011)

N′-(2-Chloro-5-nitrobenzylidene)-3-hydroxybenzohydrazide methanol solvate
por: Zhou, Zhi
Publicado: (2008)

(E)-3-Bromo-N′-(4-hydroxy-3-nitrobenzylidene)benzohydrazide
por: Cao, Guo-Biao, et al.
Publicado: (2009)

(E)-4-Chloro-N′-(5-hydroxy-2-nitrobenzylidene)benzohydrazide
por: Cao, Guo-Biao
Publicado: (2009)

Sequence-dependent quenching of fluorescein fluorescence on single-stranded and double-stranded DNA
por: Lietard, Jory, et al.
Publicado: (2022)

Differential binding of tropomyosin isoforms to actin modified with m-maleimidobenzoyl-N-hydroxysuccinimide ester and fluorescein-5-isothiocyanate
por: Skórzewski, Radosław, et al.
Publicado: (2009)

Ratiometric fluorescence imaging of cell surface pH by poly(ethylene glycol)-phospholipid conjugated with fluorescein isothiocyanate
por: Ohgaki, Ryuichi, et al.
Publicado: (2017)

Optoacoustic/Fluorescent/Acoustic Imaging Probe Based on Air-Filled Bubbles Functionalized with Gold Nanorods and Fluorescein Isothiocyanate
por: Barmin, Roman A., et al.
Publicado: (2021)

Characterization and optimization of fluorescein isothiocyanate labeling of humanized h2E2 anti-cocaine mAb
por: Kirley, Terence L., et al.
Publicado: (2023)

3-Chloro-N′-(4-hydroxy-3-nitrobenzylidene)benzohydrazide methanol disolvate
por: Zhang, Zhen
Publicado: (2011)

2-Hydroxy-N′-(2-hydroxy-3-methoxy-5-nitrobenzylidene)-3-methylbenzohydrazide
por: Han, You-Yue, et al.
Publicado: (2010)

Semisynthesis of Novel Dispiro-pyrrolizidino/thiopyrrolizidino-oxindolo/indanedione Natural Product Hybrids of Parthenin Followed by Their Cytotoxicity Evaluation
por: Singh, Chetan Paul, et al.
Publicado: (2023)

Silica particles with fluorescein-labelled cores for evaluating accessibility through fluorescence quenching by copper
por: Gallagher, Samuel H., et al.
Publicado: (2021)

Apparent endocytosis of fluorescein isothiocyanate-conjugated dextran by Saccharomyces cerevisiae reflects uptake of low molecular weight impurities, not dextran
Publicado: (1987)

Localization of antigen on lymph node dendritic cells after exposure to the contact sensitizer fluorescein isothiocyanate. Functional and morphological studies
Publicado: (1987)

High and Low Molecular Weight Fluorescein Isothiocyanate (FITC)–Dextrans to Assess Blood-Brain Barrier Disruption: Technical Considerations
por: Hoffmann, Angelika, et al.
Publicado: (2010)

Blood–brain barrier transport studies, aggregation, and molecular dynamics simulation of multiwalled carbon nanotube functionalized with fluorescein isothiocyanate
por: Shityakov, Sergey, et al.
Publicado: (2015)

Regioselectivity and diastereoselectivity of three-component reaction of α-amino acid, dialkyl acetylenedicarboxylates and 2-arylidene-1,3-indanediones
por: Chen, Liang, et al.
Publicado: (2017)

Computational study of the interaction of the psychoactive amphetamine with 1,2-indanedione and 1,8-diazafluoren-9-one as fingerprinting reagents
por: Bhikharee, Divya, et al.
Publicado: (2023)

Energy Transfer between Fluorescein Isothiocyanate and Propidium Iodide – A Problem in the Estimation of T(pot) with the Bromodeoxyuridine–DNA Flow Cytometry Technique?
por: Johansson, Maria C., et al.
Publicado: (1999)

Cannot write session to /tmp/vufind_sessions/sess_fac513fbbvbr811he1s2tkiv1f