Codon Optimization Significantly Improves the Expression Level of a Keratinase Gene in Pichia pastoris

The main keratinase (kerA) gene from the Bacillus licheniformis S90 was optimized by two codon optimization strategies and expressed in Pichia pastoris in order to improve the enzyme production compared to the preparations with the native kerA gene. The results showed that the corresponding mutation...

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Detalles Bibliográficos
Autores principales: Hu, Hong, Gao, Jie, He, Jun, Yu, Bing, Zheng, Ping, Huang, Zhiqing, Mao, Xiangbing, Yu, Jie, Han, Guoquan, Chen, Daiwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3589435/
https://www.ncbi.nlm.nih.gov/pubmed/23472192
http://dx.doi.org/10.1371/journal.pone.0058393

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3589435/
https://www.ncbi.nlm.nih.gov/pubmed/23472192
http://dx.doi.org/10.1371/journal.pone.0058393

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