Obligate Ligation-Gated Recombination (ObLiGaRe): Custom-designed nuclease-mediated targeted integration through nonhomologous end joining

Custom-designed nucleases (CDNs) greatly facilitate genetic engineering by generating a targeted DNA double-strand break (DSB) in the genome. Once a DSB is created, specific modifications can be introduced around the breakage site during its repair by two major DNA damage repair (DDR) mechanisms: th...

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Detalles Bibliográficos
Autores principales: Maresca, Marcello, Lin, Victor Guosheng, Guo, Ning, Yang, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2013
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3589542/
https://www.ncbi.nlm.nih.gov/pubmed/23152450
http://dx.doi.org/10.1101/gr.145441.112

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3589542/
https://www.ncbi.nlm.nih.gov/pubmed/23152450
http://dx.doi.org/10.1101/gr.145441.112

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