Hierarchical molecular tagging to resolve long continuous sequences by massively parallel sequencing

Here we demonstrate the use of short-read massive sequencing systems to in effect achieve longer read lengths through hierarchical molecular tagging. We show how indexed and PCR-amplified targeted libraries are degraded, sub-sampled and arrested at timed intervals to achieve pools of differing avera...

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Detalles Bibliográficos
Autores principales: Lundin, Sverker, Gruselius, Joel, Nystedt, Björn, Lexow, Preben, Käller, Max, Lundeberg, Joakim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592332/
https://www.ncbi.nlm.nih.gov/pubmed/23470464
http://dx.doi.org/10.1038/srep01186

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592332/
https://www.ncbi.nlm.nih.gov/pubmed/23470464
http://dx.doi.org/10.1038/srep01186

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