Sensitive and selective amplification of methylated DNA sequences using helper-dependent chain reaction in combination with a methylation-dependent restriction enzymes

We have developed a novel technique for specific amplification of rare methylated DNA fragments in a high background of unmethylated sequences that avoids the need of bisulphite conversion. The methylation-dependent restriction enzyme GlaI is used to selectively cut methylated DNA. Then targeted fra...

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Detalles Bibliográficos
Autores principales: Rand, Keith N., Young, Graeme P., Ho, Thu, Molloy, Peter L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592453/
https://www.ncbi.nlm.nih.gov/pubmed/22965136
http://dx.doi.org/10.1093/nar/gks831

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592453/
https://www.ncbi.nlm.nih.gov/pubmed/22965136
http://dx.doi.org/10.1093/nar/gks831

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