Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies

16S ribosomal RNA gene (rDNA) amplicon analysis remains the standard approach for the cultivation-independent investigation of microbial diversity. The accuracy of these analyses depends strongly on the choice of primers. The overall coverage and phylum spectrum of 175 primers and 512 primer pairs w...

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Detalles Bibliográficos
Autores principales: Klindworth, Anna, Pruesse, Elmar, Schweer, Timmy, Peplies, Jörg, Quast, Christian, Horn, Matthias, Glöckner, Frank Oliver
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592464/
https://www.ncbi.nlm.nih.gov/pubmed/22933715
http://dx.doi.org/10.1093/nar/gks808

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592464/
https://www.ncbi.nlm.nih.gov/pubmed/22933715
http://dx.doi.org/10.1093/nar/gks808

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