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RNA Preparation of Saccharomyces cerevisiae Using the Digestion Method may Give Misleading Results
Zymolyase (lyticase) is used for cell wall digestion in yeast experiments and is needed for incubation processes under moderate experimental conditions. This has been thought to cause unfavorable effects, and many researchers are aware that the enzyme method is unsuitable for RNA preparation followi...
| Main Authors: | , |
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| Format: | Online Article Text |
| Language: | English |
| Published: |
Springer-Verlag
2013
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| Subjects: | |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3593083/ https://www.ncbi.nlm.nih.gov/pubmed/23325148 http://dx.doi.org/10.1007/s12010-012-0051-8 |